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KMID : 0613820170270080945
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Journal of Life Science
2017 Volume.27 No. 8 p.945 ~ p.950
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Effect of Exogenous Trehalose on the Solvent Tolerance of Pseudomonas sp. BCNU 106
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Choi Hye-Jung
Lim Bo-Ra
Ha Sang-Chul
Kwon Gi-Seok
Kim Dong-Wan
Joo Woo-Hong
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Abstract
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To some extent, the growth of solvent-tolerant Pseudomonas sp. BCNU 106 is limited by toxic solvents. Therefore, various strategies to overcome this limitation need to be investigated. One such strategy is to use exogenous trehalose. The highest intracellular trehalose content of 181.88 mM was measured at 12 hr. The extracellular trehalose content decreased rapidly within 12 to 16 hr in the presence of cyclohexane. Moreover, the number of Pseudomonas sp. BCNU 106 cells grown in Luria-Bertani (LB) broth supplemented with 0.1 M trehalose in the presence of 1%(v/v) cyclohexane, hexane, propylbenzene, and m-xylene increased 89.94-, 89.72-, 91.25-, and 118.9-fold, respectively, in comparison to the control level. High survival rates of 80% and 90% were observed in the presence of cyclohexane and hexane by the addition of 0.05 M trehalose for up to 4 hr, respectively. Exogenously-added trehalose was transported into the cells, and it conferred protection against cyclohexane, hexane, propylbenzene, and m-xylene. Adding exogenous trehalose to the growth medium improved the tolerance of Pseudomonas sp. BCNU 106; thus, it is a potential biocatalyst for biotransformation and biodegradation.
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KEYWORD
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Exogenous trehalose, Pseudomonas sp., solvent tolerance, stress tolerance, trehalose
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